Περίληψη:
Renal impairment is associated with high mortality rates in severely ill
patients. The need to prevent and treat renal damage underlines the
importance of understanding the pathophysiological mechanisms that
characterize it. This could also enable early diagnosis and the design
of alternative therapeutic approaches. The aim of this study is to
investigate the effect of crocetin, a known antioxidant, on the
prevention of renal damage due to ischemia-reperfusion injury and the
investigation of the mechanisms involved. The present study was
performed on C57BL/6 mice aged 10-12 weeks. The animals had access to
water and food ad libitum. The experiment, as described in materials and
methods, was completed at 24 h, in which case the kidneys were removed
for further study, both at tissue morphology (with immunohistochemistry)
and changes in the level of miRs' expression by qRT-PCR. Accordingly,
using the automatic precision analyzer, the serum levels of the basic
parameters currently used clinically for the monitoring of renal
function were determined. The administration of crocetin, despite the
short presence of the substance in the body, affects all the biochemical
parameters analyzed (urea, creatinine, uric acid, and ions of Na, K, Cl,
P, Mg and Ca), causing significant decrease of their measured values.
Crocetin also resulted in a significant limitation of the inflammation
elements and the degree of epithelial damage. Furthermore, the
administration of crocetin appears to restore levels of expression of
miR21, miR127 and miR132.
Συγγραφείς:
Michael, Constantinos P.
Derpapas, Michael
Aravidou, Eftychia
and Sofopoulos, Michael
Michael, Panayiotis
Polydorou, Andreas
and Vezakis, Antonios
Fragulidis, Georgios P.
